齐发国际APP

孟清传授

       


       孟清,理学博士(结业于中山大先生归天学与分子生物学专业),加拿大Dalhousie大学医学院博士后,美国康内尔大学医学院初级研讨学者,瑞典卡罗林斯卡医学院、瑞典农业大学客座传授,新加坡国立大学客座传授,中国生归天学与分子生物手艺专业委员会理事,上海市学位委员会学科评断构成员,上海市生归天学与分子生物学会理事,上海市细胞学会理事,东华大先生归天学与分子生物学、基因工程仿生生物质料学传授,博士生导师。


研讨偏向:

1、功效基因中卵白质内含子的剪接机理与使用研讨。

2编码蜘蛛丝卵白质基因的克隆、表达、构造与功效、成丝机理及仿生研讨。

3、原核生物基因内含子的构造与功效、剪接与转移机理的研讨。

次要成绩:

1、掌管完成国际互助、国度天然迷信基金(项目编号:39460047)等28项科研项目。

2、揭晓研讨论文100多篇。

31986年获一项中国迷信院科技提高一等奖,1996年获一项中国农业部科技提高三等奖。

4、出书专著一部。

比年来掌管的次要科研项目:

1、上海市浦江人才设计研讨项目(项目编号:05PJ14015),2005-2007

2、国度高手艺研讨生长设计(863设计)项目(项目编号:2006AA03Z451),2006-2009

3、国度天然迷信基金面上项目(项目编号:30770463),2008-2010

4、国度天然迷信基金面上项目(项目编号:31070698)2011-2013

5、上海市根底研讨重点项目(项目编号:10JC1400300)2010-2012

6、教诲部直属高校约请外籍西席重点项目,2007-2008

7、教诲部直属高校约请外籍西席重点项目,2009-2010

8、上海市白玉兰人才基金项目(项目编号:2007B004)2007-2008

9、上海市白玉兰人才基金项目(项目编号:2009B009)2009-2010

10、教诲部博士点基金项目(课题编号:20120075110007),2013-2015

11教诲部直属高校聘专项目(1),2013-2014

12教诲部直属高校聘专项目(2),2013-2014

13教诲部直属高校学校特征项目(课题编号:TS2011DHDX025),2013-2015

14、上海市外聘专家项目,2013-2014

15)上海市科委攻关项目,(课题编号:14521100700),2014-2017

16)上海市科委国际互助项目,(课题编号:14520720200),2014-2017

17)国度天然迷信基金面上项目(项目编号:31570721),2016-2019

18)军委科技委项目(项目编号:17-163-12-ZT-003-86-01),2017-2018

19)蜘蛛丝项目,2019.01-2020.12 

比年来揭晓的次要研讨论文(*示意通讯作者)

1Zhang X1,2, Liu XQ2, Meng Q*. Engineered Ssp DnaX inteins for protein splicing with flanking proline residuesSaudi J Biol Sci. 2019 May;26(4):854-859. doi: 10.1016/j.sjbsSCI,影响因子:2.002

2Wang K1, Wen R2, Jia Q3, Liu X4, Xiao J5, Meng Q*. Analysis of the Full-Length Pyriform Spidroin Gene Sequence. Genes (Basel). 2019 Jun 3;10(6). pii: E425. doi: 10.3390/genes10060425SCI,影响因子:3.344

3Zhou Y, Rising A, Johansson J, Meng Q*. Production and properties of triple chimeric spidroins.Biomacromolecules. 2018 Apr 18. doi: 10.1021/acs.biomac.8b00402.SCI,影响因子:5.835

4Li X, Zhang XL, Cai YM, Zhang L, Lin Y, Meng Q*.Site specific labeling of two proteins in one system by atypical split inteins.Int J Biol Macromol. 2017 Nov 14. pii: S0141-8130(17)33647-4. doi: 10.1016/j.ijbiomac.2017.11.077. [Epub ahead of print]SCI,影响因子:2.502

5Rui Wena,Kangkang Wanga,Xiangqin Liub,Xue Lia,Junpeng Mia, QingMeng*a, Molecular cloning and analysis of the full-length aciniformspidroin gene from AraneusventricosusInt J Biol Macromol.https://doi.org/10.1016/ j.ijbiomac.2017.12.090SCI,影响因子:2.502

6Xu L1, Lefèvre T2, Orrell KE,Meng Q1, Auger M2, Liu XQ, Rainey JK. Structural and Mechanical Roles for the C-Terminal Nonrepetitive Domain Become Apparent in Recombinant SpiderAciniformSilk. Biomacromolecules.2017 Oct 3. doi: 10.1021/acs.biomac.7b01057.SCI,影响因子:5.835

7Wang C1, Li J1, Meng Q2, Wang B3Three Tctn proteins are functionally conserved in the regulation of neural tube patterning and Gli3 processing but not ciliogenesis and Hedgehog signaling in the mouse.Dev Biol.2017 Aug 8. pii: S0012-1606(17)30101-X. doi: 10.1016/j.ydbio.2017.08.003. [Epub ahead of print]SCI,影响因子:4.904

8Li J1, Wang C2, Wu C3, Cao T4, Xu G5, Meng Q6, Wang B7.PKA-mediated Gli2 and Gli3 phosphorylation is inhibited by Hedgehog signaling in cilia and reduced in Talpid3 mutant.Dev Biol.2017 Sep 1;429(1):147-157. doi: 10.1016/j.ydbio.2017.06.035. Epub 2017 Jul 1SCI,影响因子:4.904

9Wen R1, Liu X2, Meng Q*. Characterization of full-length tubuliformspidroin gene from Araneusventricosus.Int J Biol Macromol. 2017 Jul 19. pii: S0141-8130(17)30606-2. doi: 10.1016/j.ijbiomac.2017.07.086. [Epub ahead of print] SCI,影响因子:2.502

10Li, X., C.H. Shi, C.L. Tang, Y.M. Cai, and Q. Meng*, The correlation between the length of repetitive domain and mechanical properties of the recombinant flagelliformspidroin. Biology Open, 2017. 6(3): p. 333-339SCI,影响因子:2.9052017 Mar 15;6(3):333-339. doi: 10.1242/bio.022665.

11Landreh, M., M. Andersson, E.G. Marklund, Q.P. Jia, Q. Meng, J. Johansson, C.V. Robinson, and A. Rising, Mass spectrometry captures structural intermediates in protein fiber self-assembly. Chemical Communications, 2017. 53(23): p. 3319-3322SCI,影响因子:5.787Chem Commun (Camb). 2017 Mar 16;53(23):3319-3322. doi: 10.1039/c7cc00307b.

12Otikovs M, Andersson M, Jia Q, Nordling K, Meng Q, Andreas LB, Pintacuda G, Johansson J, Rising A, Jaudzems K1. Biomimicry of artificial spidersilk spinning assessed by NMR.Angew Chem Int Ed Engl.2017 Aug 9. doi: 10.1002/anie.201706649. [Epub ahead of print]SCI,影响因子:12.730

13Andersson, M., Q.P. Jia, A. Abella, X.Y. Lee, M. Landreh, P. Purhonen, H. Hebert, M. Tenje, C.V. Robinson, Q. Meng, G.R. Plaza, J. Johansson, and A. Rising, Biomimetic spinning of artificial spider silk from a chimeric minispidroin.NatureChemicalBiology, 2017. 13(3): p. 262.SCI,影响因子:15.808

14Lin S, Chen G, Liu X,Meng Qing*,Chimeric spider silk proteins mediated by intein result in artificial hybrid silks,Biopolymers.105(7):385-92,2016SCI,影响因子:2.572

15Dai X1,Xun Q,Liu XQ*,Meng Q*. Cysteine-free non-canonical C-intein for versatile protein C-terminal labeling through trans-splicing.Appl Microbiol Biotechnol.99(19): 8151-612015SCI,影响因子:1.879

16Xudong Dai, Xiang-Qin Liu*, Qing Meng*, Segmental expression and C-terminal labeling of protein ERp44 through protein trans-splicing, Protein Expression and Purification, 112 29–36, 2015SCI,影响因子:1.644

17Martins Otikovs, Gefei Chen, Kerstin Nordling, Michael Landreh, Qing Meng, Hans Jörnvall,NinaKronqvist, Anna Rising, Jan Johansson and Kristaps Jaudzems. Diversified structural basis of a conserved molecular mechanismfor pH dependent dimerization in spider silk N-terminal domainsChembiochem, 10.1002/cbic.2015002632015SCI,影响因子:5.838

18Marie-Laurence Tremblay1,*, Lingling Xu1,2,*, Thierry Lefèvre, MuzaddidSarker, Kathleen E. Orrell1, Jérémie Leclerc, Qing Meng, Michel Pézolet, Michèle Auger, Xiang-Qin Liu1 & Jan K. Raine, Spider wrapping silk fibre architecture arising from its modular soluble protein precursor, Scientific Reports, 26; 5: 11502,2015SCI,影响因子:4.259

19Marlene Andersson1*, Gefei Chen2*, Martins Otikovs3, Michael Landreh4, Kerstin Nordling5, Nina Kronqvist5, Per Westermark6, Hans Jörnvall4, Stefan Knight7, Yvonne Ridderstråle1, Lena Holm1, Kristaps Jaudzems3, Mitchell Chesler8, Qing Meng2*, Jan Johansson1,5,9* and Anna Rising 1,5*CO2 and H+ drive spider silk formation via opposite effects on the terminal domainsPLoS Biology, 2014, 12(8): e1001921SCI,影响因子:12.469

20Nina Kronqvist, Martins Otikovs, Volodymyr Chmyrov, Gefei Chen, Marlene Andersson, Kerstin Nordling, Michael Landreh, MédouneSarr, Hans Jörnvall, Stefan Wennmalm, Jerker Widengren, Qing Meng,Anna Rising, Daniel Otzen, Stefan D. Knight, Kristaps Jaudzems and Jan JohanssonA charge network enables pH driven sequential dimerization and stabilization of the spider silk N-terminal domainNature Communications5:3254, 2014 SCI,影响因子:12.124

21Changhua Shi, Qing Meng*, and David W. Wood*. Simple bacterial growth assay for the evalution of human thyroid hormone receptor beta mutants, Journal of Molecular Endocrinology, 52, 55-66, 2014 SCI,影响因子:3.628

22Shi C, Tarimala A, Meng Q, Wood DW. A general purification platform for toxic proteins based on intein trans-splicingAppl Microbiol Biotechnol.98(22):9425-352014,(SCI,影响因子:3.280

23Lingling Xu, Marie-Laurence Tremblay , Kathleen E. Orrellb, Jérémie Leclerc, Qing Meng*, Xiang-Qin Liu*, Jan K. Rainey*Nanoparticle self-assembly by a highly stable recombinant spider wrapping silk protein subunitFEBS Lett.587(19):3273-802013SCI,影响因子:3.601

24Ying Lin, Meng-Meng Li, Hui-Ling Song, Ling-Ling Xu, Xing-Mei Qi, Fang Hu, Qing Meng*, and Xiang-Qin Liu*Protein trans-splicing of multiple atypical split inteins engineered from natural inteinsPLoS One8(4)e595162013SCI,影响因子:4.411

25Gefei Chen, Xiangqin Liu, Yunlong Zhang, Senzhu Lin, Zijiang Yang, Jan Johansson, Anna Rising* and Qing Meng*Full-length minor ampullatespidroin sequence reveals highly variable organization of spider silk genesPLoS One7(12)e522932012SCI,影响因子:4.411

26Lingling Xu, Qing Meng*, and Xiang-Qin Liu*Silk fibers self-assembled from repetitive domain of spider wrapping silk AcSp1 proteinPLoS One711), e502272012SCI,影响因子:4.411

27Huiling Song, Qing Meng*, Xiang-Qin Liu*Protein Trans-Splicing of an Atypical Split Intein Showing Structural Flexibility and Cross-ReactivityPLoS One7(9)e453552012SCI,影响因子:4.411

28Changhua Shi,Qing Meng*,and David W. Wood*A split inteindual ELP system for recombinant protein purificationAppl Microbiol Biotechnol96, 6, 4601-32012SCI,影响因子:3.280

29Lingling Xu, Marie-Laurence Tremblay, Qing Meng, Xiang-Qin Liu, Jan K. Rainey1H, 13C and 15N NMR assignments of the aciniformspidroin(AcSp1) repetitive domain of Argiope trifasciata wrapping silk. Biomol NMR Assign, 62147-512012SCI,影响因子:1.980

30Xingmei Qi, Qing Meng*, Xiangqi Liu*Spontaneous C-cleavage of a mini-intein without its conserved N-terminal motif A. FEBS Letters585, 2513-25182011SCI,影响因子:3.601

31Qi X, Wang J, Meng Q*,Liu XQ*Alternative Nucleophilic Residues in Intein Catalysis of Protein SplicingProtein Pept Lett.18, 12, 1226-322011SCI,影响因子:1.849

32Meng Q, Zhang Y, Liu XQ. rare group I intron sequence element in a bacterial ribonucleotide reductase gene. J Bacteriol., 189, 5, 2150-21542007SCI,影响因子:4.149

33Meng Q, Wang Y, Liu XQ. An intron-encoded maturase splicing multiple introns including four group II introns in a cyanobacterial dnaN gene. J Biol Chem, 280, 42, 35085-82005SCI,影响因子:6.849

34Yang J, Meng Q, Liu XQ. Inteinharbouring large tandem repeats in replicative DNA helicase of Trichodesmiumerythraeum. Mol Microbiol, 51, 4, 1185-11922004SCI,影响因子:4.819

35Liu XQ, Yang J, Meng Q. Four inteins and three group II introns encoded in a bacterial ribonucleotide reductase gene. J Biol Chem, 278, 47, 46826-312003SCI,影响因子:6.849

36Qu Lianghu, Meng Q, Zhou H, Chen YQ. Identification of 10 novel SnoRNA gene clusters from Arabidopsis thaliana. Nucleic Acids Res, 29, 7, 1623-302001SCI,影响因子:9.112

讲授课程:

分子生物学、分子生物学手艺(为硕士研讨生开设);生物工程近况与将来(为全校本迷信生开设);生归天学与分子生物学综合实行(为生物质料本迷信生开设)。

国际交换与互助:

12002年至2004年,加拿大Dalhousie大学医学院,博士后事情;

2200612月至20072月,加拿大Dalhousie大学医学院,初级接见学者;

3201111月至20123月,美国Weill Cornell Medical College,初级接见学者;

420127月至20128月,瑞典Karolinska Institutet,初级接见学者;

520137月至20138月,瑞典Karolinska Institutet、瑞典农业大学初级接见学者;

620147月至20148月,瑞典Karolinska Institutet、瑞典农业大学初级接见学者;

720158月至20159月,美国Weill Cornell Medical College

82018715日至2018815日,加拿大Dalhousie大学医学院,初级访问学者;

9、2019年1月15日至2019年1月30日,新加坡国立大先生命迷信院,初级接见学者;

10、2019年7月12日至2019年8月12日,瑞典Karolinska Institutet,初级接见学者。  


其他信息:

联络德律风:021-67792651                                                       E-MAILmengqing@dhu.edu.cn